prl-null vector expressing renilla luciferase Search Results


prl-null vector expressing renilla luciferase  (Promega)
90
Promega prl-null vector expressing renilla luciferase
Prl Null Vector Expressing Renilla Luciferase, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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prl-null vector expressing renilla luciferase - by Bioz Stars, 2026-09
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prl-null vector  (Promega)
90
Promega prl-null vector
Prl Null Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/prl-null+vector+expressing+renilla+luciferase/prl+null+vector/pmc05207160-1022-19-21
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prl-null vector - by Bioz Stars, 2026-09
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geneporter 3000 transfection reagent  (Genlantis inc)
92
Genlantis inc geneporter 3000 transfection reagent
Geneporter 3000 Transfection Reagent, supplied by Genlantis inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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geneporter 3000 transfection reagent - by Bioz Stars, 2026-09
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capped renilla luciferase reporter rna (prl-null vector  (Promega)
90
Promega capped renilla luciferase reporter rna (prl-null vector
Capped Renilla Luciferase Reporter Rna (Prl Null Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/prl-null+vector+expressing+renilla+luciferase/capped+rna/pmc04337425-130-8-15
Average 90 stars, based on 1 article reviews
capped renilla luciferase reporter rna (prl-null vector - by Bioz Stars, 2026-09
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prlnull co-reporter vector  (Promega)
90
Promega prlnull co-reporter vector
Prlnull Co Reporter Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/prl-null+vector+expressing+renilla+luciferase/prlnull+vector/pm16491119-219-27-30
Average 90 stars, based on 1 article reviews
prlnull co-reporter vector - by Bioz Stars, 2026-09
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prlnull  (Promega)
90
Promega prlnull
Prlnull, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/prl-null+vector+expressing+renilla+luciferase/prlnull/10__1128_slash_jvi__05747___11-68-2-19
Average 90 stars, based on 1 article reviews
prlnull - by Bioz Stars, 2026-09
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prl-null  (Promega)
90
Promega prl-null
TMZ increases NF-κB transcriptional activity in MMR-proficient but not in MMR-deficient cells. ( A ) The cells were transiently co-transfected with the pNF-κB-Luc and <t>pRL-null</t> vectors, and 24 h later exposed to 50 μM TMZ+10 μM BG or to BG alone. Firefly luciferase activity was determined 48 and 72 h after drug treatment and normalized to that of <t>Renilla</t> luciferase and then to the total amount of protein used in the assays. Data are expressed in terms of fold change (i.e. the ratio between firefly luciferase activity/μg protein detected in TMZ-treated cells and that detected in the corresponding control cells). Values represent the mean of at least four independent experiments performed with duplicate samples. Bars, standard error of the mean (SEM). * p<0.05, according to paired Student’s t test analysis performed comparing firefly luciferase activity/μg protein of TMZ-treated samples with that of the corresponding controls. ( B ) M10 cells were cultured in the presence of 50 μM TMZ+10 μM BG or BG alone and culture supernatants were collected after 48 and 72 h of incubation. IL-8 and MCP-1 amount in the culture supernatants was then determined by ELISA and expressed in terms of pg protein/10 6 cells. Values represent the mean of three independent experiments performed with duplicate samples. Bars, SEM. ** p<0.01 and * p<0.05, according to Student’s t test analysis performed comparing the chemokine amount detected in the culture supernatants of TMZ-treated cells with that detected in the culture supernatants of the corresponding controls.
Prl Null, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/prl-null+vector+expressing+renilla+luciferase/prl+null/pmc03551789-77-79-84
Average 90 stars, based on 1 article reviews
prl-null - by Bioz Stars, 2026-09
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renilla control vector (prl-null  (Promega)
90
Promega renilla control vector (prl-null
TMZ increases NF-κB transcriptional activity in MMR-proficient but not in MMR-deficient cells. ( A ) The cells were transiently co-transfected with the pNF-κB-Luc and <t>pRL-null</t> vectors, and 24 h later exposed to 50 μM TMZ+10 μM BG or to BG alone. Firefly luciferase activity was determined 48 and 72 h after drug treatment and normalized to that of <t>Renilla</t> luciferase and then to the total amount of protein used in the assays. Data are expressed in terms of fold change (i.e. the ratio between firefly luciferase activity/μg protein detected in TMZ-treated cells and that detected in the corresponding control cells). Values represent the mean of at least four independent experiments performed with duplicate samples. Bars, standard error of the mean (SEM). * p<0.05, according to paired Student’s t test analysis performed comparing firefly luciferase activity/μg protein of TMZ-treated samples with that of the corresponding controls. ( B ) M10 cells were cultured in the presence of 50 μM TMZ+10 μM BG or BG alone and culture supernatants were collected after 48 and 72 h of incubation. IL-8 and MCP-1 amount in the culture supernatants was then determined by ELISA and expressed in terms of pg protein/10 6 cells. Values represent the mean of three independent experiments performed with duplicate samples. Bars, SEM. ** p<0.01 and * p<0.05, according to Student’s t test analysis performed comparing the chemokine amount detected in the culture supernatants of TMZ-treated cells with that detected in the culture supernatants of the corresponding controls.
Renilla Control Vector (Prl Null, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/prl-null+vector+expressing+renilla+luciferase/renilla+control+vector/pmc02783506-75-49-53
Average 90 stars, based on 1 article reviews
renilla control vector (prl-null - by Bioz Stars, 2026-09
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empty renilla vector (prl null)  (Promega)
90
Promega empty renilla vector (prl null)
TMZ increases NF-κB transcriptional activity in MMR-proficient but not in MMR-deficient cells. ( A ) The cells were transiently co-transfected with the pNF-κB-Luc and <t>pRL-null</t> vectors, and 24 h later exposed to 50 μM TMZ+10 μM BG or to BG alone. Firefly luciferase activity was determined 48 and 72 h after drug treatment and normalized to that of <t>Renilla</t> luciferase and then to the total amount of protein used in the assays. Data are expressed in terms of fold change (i.e. the ratio between firefly luciferase activity/μg protein detected in TMZ-treated cells and that detected in the corresponding control cells). Values represent the mean of at least four independent experiments performed with duplicate samples. Bars, standard error of the mean (SEM). * p<0.05, according to paired Student’s t test analysis performed comparing firefly luciferase activity/μg protein of TMZ-treated samples with that of the corresponding controls. ( B ) M10 cells were cultured in the presence of 50 μM TMZ+10 μM BG or BG alone and culture supernatants were collected after 48 and 72 h of incubation. IL-8 and MCP-1 amount in the culture supernatants was then determined by ELISA and expressed in terms of pg protein/10 6 cells. Values represent the mean of three independent experiments performed with duplicate samples. Bars, SEM. ** p<0.01 and * p<0.05, according to Student’s t test analysis performed comparing the chemokine amount detected in the culture supernatants of TMZ-treated cells with that detected in the culture supernatants of the corresponding controls.
Empty Renilla Vector (Prl Null), supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/prl-null+vector+expressing+renilla+luciferase/empty+renilla+vector++prl+null+/pmc05839253-74-18-21
Average 90 stars, based on 1 article reviews
empty renilla vector (prl null) - by Bioz Stars, 2026-09
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prl-null (e227a  (Promega)
90
Promega prl-null (e227a
TMZ increases NF-κB transcriptional activity in MMR-proficient but not in MMR-deficient cells. ( A ) The cells were transiently co-transfected with the pNF-κB-Luc and <t>pRL-null</t> vectors, and 24 h later exposed to 50 μM TMZ+10 μM BG or to BG alone. Firefly luciferase activity was determined 48 and 72 h after drug treatment and normalized to that of <t>Renilla</t> luciferase and then to the total amount of protein used in the assays. Data are expressed in terms of fold change (i.e. the ratio between firefly luciferase activity/μg protein detected in TMZ-treated cells and that detected in the corresponding control cells). Values represent the mean of at least four independent experiments performed with duplicate samples. Bars, standard error of the mean (SEM). * p<0.05, according to paired Student’s t test analysis performed comparing firefly luciferase activity/μg protein of TMZ-treated samples with that of the corresponding controls. ( B ) M10 cells were cultured in the presence of 50 μM TMZ+10 μM BG or BG alone and culture supernatants were collected after 48 and 72 h of incubation. IL-8 and MCP-1 amount in the culture supernatants was then determined by ELISA and expressed in terms of pg protein/10 6 cells. Values represent the mean of three independent experiments performed with duplicate samples. Bars, SEM. ** p<0.01 and * p<0.05, according to Student’s t test analysis performed comparing the chemokine amount detected in the culture supernatants of TMZ-treated cells with that detected in the culture supernatants of the corresponding controls.
Prl Null (E227a, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/prl-null+vector+expressing+renilla+luciferase/prl+null++e227a/pmc09356134-117-25-27
Average 90 stars, based on 1 article reviews
prl-null (e227a - by Bioz Stars, 2026-09
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renilla reniformis vector prl-null  (Promega)
90
Promega renilla reniformis vector prl-null
TMZ increases NF-κB transcriptional activity in MMR-proficient but not in MMR-deficient cells. ( A ) The cells were transiently co-transfected with the pNF-κB-Luc and <t>pRL-null</t> vectors, and 24 h later exposed to 50 μM TMZ+10 μM BG or to BG alone. Firefly luciferase activity was determined 48 and 72 h after drug treatment and normalized to that of <t>Renilla</t> luciferase and then to the total amount of protein used in the assays. Data are expressed in terms of fold change (i.e. the ratio between firefly luciferase activity/μg protein detected in TMZ-treated cells and that detected in the corresponding control cells). Values represent the mean of at least four independent experiments performed with duplicate samples. Bars, standard error of the mean (SEM). * p<0.05, according to paired Student’s t test analysis performed comparing firefly luciferase activity/μg protein of TMZ-treated samples with that of the corresponding controls. ( B ) M10 cells were cultured in the presence of 50 μM TMZ+10 μM BG or BG alone and culture supernatants were collected after 48 and 72 h of incubation. IL-8 and MCP-1 amount in the culture supernatants was then determined by ELISA and expressed in terms of pg protein/10 6 cells. Values represent the mean of three independent experiments performed with duplicate samples. Bars, SEM. ** p<0.01 and * p<0.05, according to Student’s t test analysis performed comparing the chemokine amount detected in the culture supernatants of TMZ-treated cells with that detected in the culture supernatants of the corresponding controls.
Renilla Reniformis Vector Prl Null, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/prl-null+vector+expressing+renilla+luciferase/renilla+reniformis/pm29519853-41-32-36
Average 90 stars, based on 1 article reviews
renilla reniformis vector prl-null - by Bioz Stars, 2026-09
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prl-null luciferase protein expression plasmid  (Promega)
90
Promega prl-null luciferase protein expression plasmid
TMZ increases NF-κB transcriptional activity in MMR-proficient but not in MMR-deficient cells. ( A ) The cells were transiently co-transfected with the pNF-κB-Luc and <t>pRL-null</t> vectors, and 24 h later exposed to 50 μM TMZ+10 μM BG or to BG alone. Firefly luciferase activity was determined 48 and 72 h after drug treatment and normalized to that of <t>Renilla</t> luciferase and then to the total amount of protein used in the assays. Data are expressed in terms of fold change (i.e. the ratio between firefly luciferase activity/μg protein detected in TMZ-treated cells and that detected in the corresponding control cells). Values represent the mean of at least four independent experiments performed with duplicate samples. Bars, standard error of the mean (SEM). * p<0.05, according to paired Student’s t test analysis performed comparing firefly luciferase activity/μg protein of TMZ-treated samples with that of the corresponding controls. ( B ) M10 cells were cultured in the presence of 50 μM TMZ+10 μM BG or BG alone and culture supernatants were collected after 48 and 72 h of incubation. IL-8 and MCP-1 amount in the culture supernatants was then determined by ELISA and expressed in terms of pg protein/10 6 cells. Values represent the mean of three independent experiments performed with duplicate samples. Bars, SEM. ** p<0.01 and * p<0.05, according to Student’s t test analysis performed comparing the chemokine amount detected in the culture supernatants of TMZ-treated cells with that detected in the culture supernatants of the corresponding controls.
Prl Null Luciferase Protein Expression Plasmid, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/prl-null+vector+expressing+renilla+luciferase/prl+null+luciferase+protein+expression+plasmid/us10053671-858-46-51
Average 90 stars, based on 1 article reviews
prl-null luciferase protein expression plasmid - by Bioz Stars, 2026-09
90/100 stars
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Image Search Results


TMZ increases NF-κB transcriptional activity in MMR-proficient but not in MMR-deficient cells. ( A ) The cells were transiently co-transfected with the pNF-κB-Luc and pRL-null vectors, and 24 h later exposed to 50 μM TMZ+10 μM BG or to BG alone. Firefly luciferase activity was determined 48 and 72 h after drug treatment and normalized to that of Renilla luciferase and then to the total amount of protein used in the assays. Data are expressed in terms of fold change (i.e. the ratio between firefly luciferase activity/μg protein detected in TMZ-treated cells and that detected in the corresponding control cells). Values represent the mean of at least four independent experiments performed with duplicate samples. Bars, standard error of the mean (SEM). * p<0.05, according to paired Student’s t test analysis performed comparing firefly luciferase activity/μg protein of TMZ-treated samples with that of the corresponding controls. ( B ) M10 cells were cultured in the presence of 50 μM TMZ+10 μM BG or BG alone and culture supernatants were collected after 48 and 72 h of incubation. IL-8 and MCP-1 amount in the culture supernatants was then determined by ELISA and expressed in terms of pg protein/10 6 cells. Values represent the mean of three independent experiments performed with duplicate samples. Bars, SEM. ** p<0.01 and * p<0.05, according to Student’s t test analysis performed comparing the chemokine amount detected in the culture supernatants of TMZ-treated cells with that detected in the culture supernatants of the corresponding controls.

Journal: Journal of Translational Medicine

Article Title: NF-κB is activated in response to temozolomide in an AKT-dependent manner and confers protection against the growth suppressive effect of the drug

doi: 10.1186/1479-5876-10-252

Figure Lengend Snippet: TMZ increases NF-κB transcriptional activity in MMR-proficient but not in MMR-deficient cells. ( A ) The cells were transiently co-transfected with the pNF-κB-Luc and pRL-null vectors, and 24 h later exposed to 50 μM TMZ+10 μM BG or to BG alone. Firefly luciferase activity was determined 48 and 72 h after drug treatment and normalized to that of Renilla luciferase and then to the total amount of protein used in the assays. Data are expressed in terms of fold change (i.e. the ratio between firefly luciferase activity/μg protein detected in TMZ-treated cells and that detected in the corresponding control cells). Values represent the mean of at least four independent experiments performed with duplicate samples. Bars, standard error of the mean (SEM). * p<0.05, according to paired Student’s t test analysis performed comparing firefly luciferase activity/μg protein of TMZ-treated samples with that of the corresponding controls. ( B ) M10 cells were cultured in the presence of 50 μM TMZ+10 μM BG or BG alone and culture supernatants were collected after 48 and 72 h of incubation. IL-8 and MCP-1 amount in the culture supernatants was then determined by ELISA and expressed in terms of pg protein/10 6 cells. Values represent the mean of three independent experiments performed with duplicate samples. Bars, SEM. ** p<0.01 and * p<0.05, according to Student’s t test analysis performed comparing the chemokine amount detected in the culture supernatants of TMZ-treated cells with that detected in the culture supernatants of the corresponding controls.

Article Snippet: HCT116/3-6, HCT116, M10, pUSE2 and KD12 cells were seeded into 24-well plates (BD Falcon™, Becton, Dickinson and Company, Franklin Lakes, NJ) and allowed to adhere at 37°C for 18 h. The cells were then transiently co-transfected with 3 μg (M10) or 0.075 μg (HCT116/3-6, HCT116) or 0.3 μg (pUSE2 and KD12) of the NFκB-responsive firefly luciferase reporter pNF-κB-Luc (Stratagene, La Jolla, CA) and 250 ng (M10) or 6.25 ng (HCT116/3-6, HCT116) or 25 ng (pUSE2 and KD12) of the Renilla luciferase expression vector pRL-null (Promega Corporation, Madison, WI) using Lipofectamine™ 2000 Reagent (Invitrogen Corporation) according to the manufacturer’s protocol.

Techniques: Activity Assay, Transfection, Luciferase, Cell Culture, Incubation, Enzyme-linked Immunosorbent Assay